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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -BAFF (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1, THANK) (Biotin)

Anti -BAFF (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1, THANK)
(Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF-and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF-kB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion. Two receptors for BAFF were recently identified and designated TACI and BCMA. BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases. Like TNFa and TRAIL, THANK was shown to activate NF-kB and c-jun N-terminal kinase (JNK) and to induce apoptosis.
Catalog #B0003-64S
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionDirect ELISA: 1ug/ml. Using 100ul/well allows the detection of 0.2-0.4ng/well of recombinant human BAFF.
Sandwich ELISA: 0.25-1ug/ml. Using 100ul/well allows the detection of 0.2-0.4ng/well of recombinant human BAFF.
Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant human BAFF is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2. Labeled with Biotin. Reconstitute with 50ul sterile PBS, 0.1% BSA.
PurityPurified by affinity chromatography.
ImmunogenRecombinant protein corresponding to human BAFF, expressed in E. coli.
SpecificityRecognizes human BAFF.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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