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BAFF, Recombinant, Human (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1, THANK)
BAFF, Recombinant, Human (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1,
THANK)
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| Human BAFF (for B cell activating factor belonging to the TNF family) is a newly discovered novel ligand of the TNF family. BAFF plays an important role as costimulator of B cell proliferation and function. Recombinant Human BAFF is a soluble 17kD protein containing 153 amino acid residues, comprising the TNF-like extra-celluar domain of BAFF. | | | Catalog # | B0003-66 | | Biological Activity | Assay#1: Determined by the dose-dependent stimulation of IL-8 production by Human PBMC (Peripheral Blood Mononuclear Cells). The ED50 for this effect is 5-10ng/ml. Please Note: Results may vary with PBMC donors. | | Assay#2: Determined by a mouse splenocyte survival assay. The expected ED50 for this effect is 0.5-2.0ug/ml. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Country of Origin | USA | | Source | E. coli | | Purity | 95% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug). | | Form | Supplied as a sterile filtered, lyophilized powder from PBS, pH 7.5, 0.5 DTT. Reconstitute in dH2O to a concentration of 0.1-1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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