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BAFF, Recombinant, Human (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1, THANK)
BAFF, Recombinant, Human (B cell Activating Factor belonging to TNF Family, BLyS, TALL-1,
THANK)
Pricing
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| Catalog # | B0003-66B | | Source | Recombinant corresponding to 153aa from human BAFF expressed in E. coli. | | Molecular Weight | ~17kD | | Endotoxin | ~0.1ng/ug | | Biological Activity | ED50=5-10ng/ml, determined by the dose dependent stimulation of IL-8 production by human PBMCs. Please note that this biological activity may vary with each PBMC donor. The biological activity was also determined in a mouse splenocyte survival assay. The ED50 for this assay is 0.5 - 2.0 µg/mL. BAFF is a member of the TNF ligand superfamily, which serves as a costimulator of B cell proliferation and function. This recombinant protein corresponds to the TNF-like portion of BAFF’s extracellular domain. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~17kD | | Source | E. coli. | | Purity | ~95% (SDS PAGE, HPLC) | | Concentration | Not determined | | Form | Lyophilized from 5 mM Tris, pH 8.0, plus 3 mM NaCl. Reconstitute with sterile dH2O to 0.1-1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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