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Specifications

The human B cell activating factor (BAFF) and APRIL(a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily (TNFSFL #13b and 13 respectively). They are expressed by non-B cells, and are down regulated by mitogenic stimulation(2). BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells (3,4). Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells(1). Recently a third receptor for BAFF (BAFF-R) was described(5). BAFF and BAFFR knockout mice have a reduced numbers of mature B cells in the periphery, however TACI and BCMA knockouts do not share this phenotype, suggesting that BAFF-R may the primary receptor for BAFF in mice (8,9,10). Cell surface BAFF can be proteolytically cleaved to form a soluble trimeric molecule (2). Levels of soluble BAFF correspond with levels of autoantibodies in Sjogren's Syndrome(11). Recombinant BAFF(trn)-muCD8 binds to human cell surface TACI/BCMA/BAFFR.
Catalog #	B0003-67E
Functional Application	Human BAFF(trn)-muCD8 binds to human cell surface TACI/BCMA/BAFFR .
	Human BAFFtrn (TALL-1, Blys, THANK)-muCD8 Fusion Protein*
Molecular Structure	A soluble molecule consisting of 151 (VQGPEET...c-term) aminoacids of the extracellular domain of human BAFF fused to the extracellular domain (167aa) of murine CD8 alpha, with a predicted monomeric weight of 38.5kD. A portion of the extracellular region of BAFF was truncated to eliminate a potential protease cleavage site.
Transfectant Cell Line	CHO
Applications	Suitable for use in Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight	38.5kD
Source	CHO cells
Purity	Purified by affinity chromatography.
Concentration	~0.5mg/ml
Form	Supplied as a liquid in 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 0.5mg/ml Gentamicin Sulfate.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.