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BAFF Receptor IgG2a Fc, Fusion Protein (BAFF-R, BR3, BLyS Receptor 3)
BAFF Receptor IgG2a Fc, Fusion Protein (BAFF-R, BR3, BLyS Receptor 3)
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| The human B cell activating factor (BAFF) and APRIL(a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily. They are expressed by non-B cells, and are down regulated by mitogenic stimulation(2). BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells(3,4). Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells(1). A third receptor for BAFF (BAFF-R) has been described(5). BAFF and BAFFR knockout mice have a reduced numbers of mature B cells in the periphery, however TACI and BCMA knockouts do not share this phenotype, suggesting that BAFF-R may the primary receptor for BAFF in mice(8,9,10). Cell surface BAFF can be proteolytically cleaved to form a soluble trimeric molecule(2). Levels of soluble BAFF correspond with levels of autoantibodies in Sjogren's Syndrome(11). Recombinant human BAFFR-muIg binds to recombinant BAFF-muCD8 and can inhibit binding of this molecule to receptors on Raji cells. | | | Catalog # | B0003-68J | | Molecular Structure | A soluble molecule consisting of the extracellular (60aa) domain of human BAFFR fused to the murine IgG2a Fc (232 aa). Predicted monomeric weight: 33. 5kD. | | Transfectant Cell Line | CHO | | Functional Application | BAFFR-muIg binds to recombinant BAFF-muCD8 and can inhibit this molecule's abiltiy to bind to receptors on Raji cells. | | Applications | Suitable for use in ELISA. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | | | | Molecular Weight | 33.5kD | | Source | CHO cells | | Purity | Purified by Protein A affinity chromatography. | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, 0.5mg/ml gentamicin sulfate. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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