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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -BAFF Receptor (BAFFR, BAFF-R, B Cell-activating Factor Receptor, BLyS Receptor 3, BR3, BROMIX, CD268, CVID4, MGC138235, Prolixin, Tumor Necrosis Factor Receptor Superfamily Member 13C, TNFRSF13C) (Biotin)

Anti -BAFF Receptor (BAFFR, BAFF-R, B Cell-activating Factor Receptor, BLyS Receptor 3,
BR3, BROMIX, CD268, CVID4, MGC138235, Prolixin, Tumor Necrosis Factor Receptor Superfamily
Member 13C, TNFRSF13C) (Biotin)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
Human CD268 (BAFFR, BAFF receptor) is a type I TNF superfamily receptor member #13c. It is highly specific for CD257 (BAFF), which itself does bind to other receptors TACI, and BCMA. CD268 expressed on B cells and its ligation by CD257 (BAFF) regulates maturation.
Catalog #B0003-68Y2
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. Wash 5x10e5 cultured human Raji cells per tube and incubated 45 minutes on ice with 80ul of B0003-68Y2 at 10ug/ml.
2. Cells were washed twice and incubated with secondary reagent Streptavidin/R-PE (S7973-88P) after which they were washed three times, fixed and analyzed by FACS.
3. Cells stained positive with a mean shift of 1.1 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration.
3. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml B0003-68Y.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No11K1
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
PurityPurified by Protein A affinity chromatography
ImmunogenRecombinant protein corresponding to human BAFF Receptor
SpecificityRecognizes human BAFF Receptor Binds to recombinant CD268 (but not CD269) in ELISA and native CD268 expressed on Raji cell surface.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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