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You are here:Home » Antibodies » Abs to Enzymes, Glycosyltransferase » Anti -B3GNT1 (N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase, I-beta-1,3-N-acetylglucosaminyltransferase, iGnT, Poly-N-acetyllactosamine Extension Enzyme, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1, B3GNT6)

Anti -B3GNT1 (N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase,
I-beta-1,3-N-acetylglucosaminyltransferase, iGnT, Poly-N-acetyllactosamine Extension
Enzyme, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1, B3GNT6)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
B3GNT1 is a 50kD member of the b3GnT family of enzymes. It is synthesized by sensory neurons, endothelial cells and skeletal muscle. In the trans-Golgi, it collaborates with B4GALT1 to generate a linear poly-N-acetyllactosamine sequence that can be incorporated into either N-linked or O-linked glycans. Human B3GNT1 is a 451aa type II transmembrane protein. It contains an 8aa N-terminal cytoplasmic segment, an extended 28aa transmembrane region aa9-36, and a 379aa luminal domain aa37-415. Over aa37-415, human B3GNT1 shares 97% aa identity with mouse B3GNT1.
Catalog #B0010-50
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2B
Clone No12K14
HostMouse
SourceHuman
Concentration~0.5mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant corresponding to aa43-415 from human B3GNT1 expressed in CHO cell line.
SpecificityRecognizes human B3GNT1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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