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You are here:Home » Culture Media » Media-Microbiological » Baird Parker Agar (Powder)

Baird Parker Agar (Powder)

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Specifications

Selective isolation of coagulase positive Staphylococci in food.
Catalog #B0050
DescriptionBaird-Parker modified the Tellurite Glycine Medium of Zebovitz, Evans and Niven. It is recommended for microbial limit tests by the USP. Potassium Tellurite and Lithium Chloride selectively suppresses the growth of most bacteria but allows the growth of Staphylococcus aureus. The addition of pyruvate and glycine enhances the growth of Staphylococci. The differentiation of coagulase-positive staphylococci is noted by the reduction of Potassium Tellurite which results in a black colony. Staphylococcus that contain lecithinase break down the egg yolk and cause a clear zone. An opaque zone may be formed due to lipase activity.
ComponentsShown as g/liter
Casein Digest Peptone 10.0
Lithium Chloride 5.0
Beef Extract 5.0
Agar 17.0
Yeast Extract 1.0
Glycine 12.0
Sodium Pyruvate 10.0
Total: 60g/liter
DirectionsDissolve 60g. in 800ml of ddH2O, heating to boiling with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi). After cooling to 45-50°C add 10ml of a sterile I% Potassium Tellurite solution and 50ml of sterile egg yolk emulsion.
Storage and StabilityStore powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Microbiological Analysis
Bacillus subtilis (ATCC 6633)Inhibited
Escherichia faecalis (ATCC 29212)Excellent Black
Escherichia Coli (ATCC 25922)
Inhibited
Proteus mirabilis (ATCC 12453)Good Brown
Staphylococcus aureus (ATCC 6538)
Excellent Black
Staphylococcus aureus (ATCC 25923)Excellent Black
Staphylcoccus epidermidis (ATCC 12228)Fair Grey/ black
Appearance
Light beige, homogeneous and free flowing
Solubility
Light to medium amber and opaque.
Canary yellow with the egg yolk addition
pH7.0 ± 0.2
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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