Anti -Bak, NT (BAK1, BCL2-antagonist/killer 1, BAK, CDN1, BCL2L7, cell death inhibitor 1,
apoptosis regulator BAK, Bcl-2 homologous antagonist/killer)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IH IC FC |
|
| Bak a pro-apoptotic analog to Bax, is a member of the Bcl-2 family of proteins. During mitochondria-regulated programmed cell death, Bak promotes apoptosis by binding Bcl-2 and inhibiting its anti-apoptotic function (1). Upon apoptotic stimulation, Bak translocates from the cytosol to the mitochondrial membrane (2). Pro-apoptotic cascade activates BID, which oligomerizes Bak into pores that result in the release of cytochrome c (2, 3). In healthy cells, Bak associate with Bcl-xL and Mcl-1 but not with Bcl-2, Bcl-w or A1 and only when freed of both Bak will induce apoptosis. | | | Catalog # | B0055-10A | | Applications | Suitable for use in Flow Cytometry, Western Blotting, Immunohistochemistry, Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1:10 | | Western Blotting: 1:1,000-5,000 | | Immunohistochemistry: 1:250-500 | | Immunocytochemistry: 1:250-500 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 6k35 | | Host | Rabbit | | Source | Human | | Form | Supplied as a liquid | | Purity | Supernatant | | Immunogen | A synthetic peptide corresponding to residues near the N-term of human Bak | | Specificity | Recognizes human Bak | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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