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You are here:Home » Antibodies » Antibodies-Cancer Markers » Anti -BARD1 (BRCA1 Associated Ring Domain 1)

Anti -BARD1 (BRCA1 Associated Ring Domain 1)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
BARD1 possesses an N-terminal RING motif and the C-terminal BRCT domain. As the name suggests, this protein is the major cellular binding protein of BRCA1. BARD1 is thought to play a key role in regulating BRCA1 stability, localisation and function. BARD1 is also reported to function as a tumor suppressor, since mutations in this protein have been implicated in a number of breast and ovarian cancers.
Catalog #B0085-01
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:15000
Immunoprecipitation: 10-40ug/mg protein
Optimal dilution determined by the researcher.
Recommended Secondary ReagentsI1903-30U IgG, H&L, X-adsorbed (HRP) Sheep anti rabbit
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in Tris-citrate/phosphate, pH 7.4, 0.09% sodium azide before the addition of glycerol to 40%.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to a region of human BARD1 encoded within exon 4.
SpecificityRecognizes human BARD1 (BRCA1 associated Ring Domain 1).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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