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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -BARD1 (BRCA1 Associated Ring Domain 1)

Anti -BARD1 (BRCA1 Associated Ring Domain 1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP Gel
BARD1 interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, BARD1 shares homology with the 2 most conserved regions of BRCA1: the N-terminal ring motif and the C-terminal BRCT domain. The ring motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. The BARD1 protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interation is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. BARD1 may be the target of oncogenic mutations in breast or ovarian cancer.
Catalog #B0085-01C
ApplicationsSuitable for use in Dot Blot, Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionWestern Blot: 0.2-2ug/ml.
Immunoprecipitation: 100-500ug/sample
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8C29
HostMouse
SourceHuman
Concentration0.05mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa50-200 from the internal region of human BARD1.
SpecificityRecognizes human BARD1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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