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You are here:Home » Antibodies » Antibodies-Connexin, Junction Proteins » Anti -Barmotin (Tight Junction-associated Protein)

Anti -Barmotin (Tight Junction-associated Protein)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Tight junctions function to maintain cellular polarity and permeability barriers in epithelial and endothelial cells. Several molecules of the tight junction complex have been identified. Studies with Madin-Darby canine kidney (MDCK) cells indicate the 7H6 antigen to be an important molecule for regulation of the barrier function of tight junctions. Confocal laser scanning microscopy revealed that the expression of 7H6 antigen decreased sequentially during hepatocarcinogenesis. The 7H6 antigen was expressed intensely at the apical and basolateral membrane of cancer cells with glandular pattern. Examination of human gastric and colon cancer tissues showed that tight junctions were maintained highly in the well-differentiated (gland-forming) adenocarcinomas, but reduced in poorly-differentiated adenocarcinomas. In MDCK cells the disappearance of 7H6 expression is closely related with cell spreading in vitro induced hepatocyte growth factor/scatter factor.
Catalog #B0094-17
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:10
Immunohistochemistry (Frozen): 1:10
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No7H6
FormSupplied as a liquid in PBS, 0.1% BSA.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman Barmotin
SpecificityRecognizes human Barmotin at ~155kD. Localized at the periphery of tight junctions of hepatocytes and other epithelia and in the intestine and kidney. Species Crossreactivity: Canine, mouse, rat and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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