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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Bas (2091-2490), protein tyrosine phosphatase, catalytic domain, Recombinant, Human (PTPL1, FAP-1, PTPN13)

Bas (2091-2490), protein tyrosine phosphatase, catalytic domain, Recombinant, Human
(PTPL1, FAP-1, PTPN13)

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Specifications

Catalog #B0099
Catalytic domain(a.a. 2091-2490), .
Specific Activity~1 U/ug.
Unit DefinitionOne unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30 °C
ApplicationSuitable for use in the study of enzyme kinetics, regulation and to dephosphorylate target substrates.
Enzyme Assay Buffer and Substrate50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50mM pNPP.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Molecular Weight71.8kD
SourceRecombinant human, N terminal GST tag in E. coli.
Purity90% by SDS-PAGE.
FormSupplied as a liquid in 25mM Tris-HCl, pH 8.0, 75mM sodium chloride, 0.05% Tween-20, 50% glycerol, 2mM EDTA, 1mM DTT, 10mM glutathione.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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