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You are here:Home » Antibodies » Abs to Keratin, Cytokeratin » Anti -Basic Hair Keratin, K86 (KRT86, Keratin 86, FLJ25176, Hb1, Hb6, HB6, hHb6, K2.11, K86, Keratin, type II cuticular Hb6, Keratin-86, KRTHB1, KRTHB6, MNX, Type II Hair Keratin Hb6)

Anti -Basic Hair Keratin, K86 (KRT86, Keratin 86, FLJ25176, Hb1, Hb6, HB6, hHb6, K2.11,
K86, Keratin, type II cuticular Hb6, Keratin-86, KRTHB1, KRTHB6, MNX, Type II Hair Keratin
Hb6)

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Specifications

Clone Host Grade Applications
Polyclonal Guinea pig Serum B IH
Catalog #B0130-32
ApplicationsSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (frozen): 1:200. Preincubate fixed sections with 0.1 Triton X-10 (in PBS) for 1-5 min prior to 1 hour incubation.
Western Blot: 1:2000. Incubate for 1 hour at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGuinea pig
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid, 0.09% sodium azide.
PuritySerum
ImmunogenSynthetic peptide corresponding to human basic hair keratin K86, APV VST RVS SVP SNS NVV VGT TNA C (KLH).
SpecificityRecognizes human hair keratin (hHb6) expressed starting from the middle hair cortex. It can be defect in monilethrix. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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