Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -Bassoon

Anti -Bassoon

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IF
Bassoon is a 420kD protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96%. The human BASSOON gene has recently been cloned and localized. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors. Another presynaptic protein, Piccolo was found to colocalize with Bassoon in cultured hippocampal neurons. These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release.
Catalog #B0140-01A
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionsImmunofluorescence (IC): 1:400
Immunohistochemistry (Paraffin): 1:10-1:2000
Immunoprecipitation: 2.5ug
Western Blot: 1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No8J289
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant rat Bassoon
SpecificityRecognizes rat bassoon at ~416kD. Additional bands between 97kD and 400kD have been identified and are reported to be proteolytic degradation products of bassoon. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links