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B0290-05 Rabbit Anti-BCKDHA, CT (2-oxoisovalerate Dehydrogenase Subunit alpha, Mitochondrial, Branched-chain alpha-keto Acid Dehydrogenase E1 Component alpha Chain, BCKDH E1-alpha, BCKDE1A)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P12694
Isotype
IgG
Grade
Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_000700.1
Shipping Temp
Blue Ice
Storage Temp
-20°C

The branched-chain alpha-keto acid (BCAA) dehydrogenase(BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). BCKDHA is the alpha subunit of the decarboxylase (E1) component.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human BCKDHA (KLH).
Form
Supplied as a liquid in PBS, 0.123% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human BCKDHA.
References
1. Flaschker,N., et.al., J. Inherit. Metab. Dis. 30(6), 903-909 (2007).
USBio References
No references available
Conjugates
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