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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant IH

BCA-225, a 225kD glycoprotein, is secreted by the T47D breast carcinoma cell line. Strong intracytoplasminc staining is seen in primary and metastatic breast carcinoma cell lines, primary and metastatic breast carcinoma tissue and cervical carcinomas.
Catalog #	B0395-10B
Application	Suitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry (formalin fixed paraffin sections): 1:40 or higher for biotin-based detection systems. Incubate 20 mins. at RT. Tissue pre-treatment not required.
	Optimal dilution to be determined by the researcher.
Positive Control	Human breast carcinoma
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	6A9
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Purity	Tissue culture supernatant
Immunogen	Protein secreted by the T47D (Clone 11) human breast carcinoma cell line.
Specificity	Recognizes human BCA-225.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.