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You are here:Home » Antibodies » Abs to B Cells » Anti -B Cell Linker Protein (BLNK, BASH, BLNK-s, Ly57, MGC111051, SLP-65, SLP65)

Anti -B Cell Linker Protein (BLNK, BASH, BLNK-s, Ly57, MGC111051, SLP-65, SLP65)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified B
BLNK plays a pivotal role in B cell activation through cross-linkage of the B-cell Receptor (BCR), leading to the activation of protein tyrosine kinases (PTKs) including Syk. The resulting tyrosine phosphorylation of BLNK by Syk, provides docking sites for the SH2-containing effector molecules such as Vav, PLC gamma and the adaptor proteins Crb2 and Nck, thereby acting as a central linker protein in many different B cell signalling pathways involved in proliferation, differentiation and apoptosis.
Catalog #B0397-10A
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:2000, detects two bands of approximately 68 and 70kD in mouse Sp2/0 cell lysates.
Optimal dilution determined by the researcher.
Recommended Secondary ReagentsI1903-30U IgG, H&L, X-adsorbed (HRP)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
PurityPurified by ammonium sulfate precipitation.
ImmunogenFusion protein corresponding to amino acids 171-356 of human BLNK.
SpecificityRecognizes B-cell Linker Protein (BLNK), a cytosolic adaptor molecule, visualised as two phosphoproteins of 68 and 70kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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