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You are here:Home » Antibodies » Abs to B Cells » Anti -B Cell Linker Protein, phosphorylated (Tyr96) (BLNK, BASH, BLNK-s, Ly57, MGC111051, SLP-65, SLP65)

Anti -B Cell Linker Protein, phosphorylated (Tyr96) (BLNK, BASH, BLNK-s, Ly57, MGC111051,
SLP-65, SLP65)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
BLNK (B cell linker protein) is an adaptor molecule that plays key roles from B cell activation through B cell antigen receptor (BCR) engagent. BLNK acts on the inferface between BCR-associated Syk and the downstream signaling cascade (1,2). BLNK has multiple SH2 binding motifs (YXXP) at its N-terminus and an SH2 domain at its C-terminus. After BCR ligation, BLNK is phosphorylated by Syk on its multiple YXXP motifs including Tyr72, Tyr84, Tyr96 and Tyr178 (1). The phosphorylation on these motifs provides docking sites for signaling molecules such as Btk, PLCgamma and Vav. They bind to BLNK through their SH2 domains and cooperatively activate downstream signaling (3,4). BLNK can also interact with phosphorylated targets such as HPK1 through its SH2 domain to activate the pathway (5).
Catalog #B0397-15
ApplicationsSuitable for use in Western Blotting, Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
PurityPurified by protein A and peptide affinity chromatography.
ImmunogenSynthetic phosphopeptide (KLH coupled) corresponding to residues around Tyr96 of human BLNK.
SpecificityDetects endogenous levels of Tyr96 phosphorylated BLNK.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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