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You are here:Home » Antibodies » Abs to Cells, Blood Components » Anti -B Cells (FITC)

Anti -B Cells (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified FC
Catalog #B0401
Studies on normal lymphocytes, leukemic cells and cell lines indicate it is a marker for a limited segment of the B cell maturation pathway(2,3). This glycoprotein is expressed in varying degrees on normal circulating B-cells, chronic B-cell leukemia (B-CLL), prolymphocytic leukemia (PLL) and other B cell neoplasias (41)2. It labels the same population as CD22 antibodies but reacts with a different antigen(5). Cell reactivity Stains peripheral blood B lymphocytes and tonsil B lymphocytes. (1).
This antibody can be used for the differentiation of PLL from B-CLL. It is also useful in confirmation of the diagnosis of other disorders such as HCL, HCL-V and SLVL. Useful for the detection of residual disease in patients undergoing treatment for these disorders.
ApplicationsSuitable for use in Flow Cytometry. Other applications have not been tested.
Recommended DilutionFlow Cytometry: Neat. Use 10ul per 100ul of whole blood, or 1x10e6 peripheral blood mononuclear cells (PBMC) in 100ul buffer.
Optimal dilution to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No2Q385
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 0.1M Tris-HCl, 0.5M sodium chloride, 10mM glycine, pH 8.0, 0.2% BSA, 0.1% sodium azide.
PurityPurified ascites. Tested by electrophoresis and Flow Cytometry.
ImmunogenThe human B-lymphoblastoid cell line, HRIK.
SpecificityDetects a glycoprotein of 105kD found on circulating B-lymphocytes. Reacts with HRIK and Raji cell lines. Clinical Expression
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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