| Catalog # | B0401 |
| Studies on normal lymphocytes, leukemic cells and cell lines indicate it is a marker for a limited segment of the B cell maturation pathway(2,3). This glycoprotein is expressed in varying degrees on normal circulating B-cells, chronic B-cell leukemia (B-CLL), prolymphocytic leukemia (PLL) and other B cell neoplasias (4–1)2. It labels the same population as CD22 antibodies but reacts with a different antigen(5). Cell reactivity | Stains peripheral blood B lymphocytes and tonsil B lymphocytes. (1). |
| This antibody can be used for the differentiation of PLL from B-CLL. It is also useful in confirmation of the diagnosis of other disorders such as HCL, HCL-V and SLVL. Useful for the detection of residual disease in patients undergoing treatment for these disorders. |
| Applications | Suitable for use in Flow Cytometry. Other applications have not been tested. |
| Recommended Dilution | Flow Cytometry: Neat. Use 10ul per 100ul of whole blood, or 1x10e6 peripheral blood mononuclear cells (PBMC) in 100ul buffer. |
| Optimal dilution to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgM |
| Clone No | 2Q385 |
| Host | Mouse |
| Source | Human |
| Concentration | 100tests |
| Form | Supplied as a liquid in 0.1M Tris-HCl, 0.5M sodium chloride, 10mM glycine, pH 8.0, 0.2% BSA, 0.1% sodium azide. |
| Purity | Purified ascites. Tested by electrophoresis and Flow Cytometry. |
| Immunogen | The human B-lymphoblastoid cell line, HRIK. |
| Specificity | Detects a glycoprotein of 105kD found on circulating B-lymphocytes. Reacts with HRIK and Raji cell lines. Clinical Expression |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
