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You are here:Home » BioSeparation? » BioSep-Assay Detection, Substrates » BCIP/NBT Stable Liquid Substrate 0.577mM BCIP/0.122mM NBT

BCIP/NBT Stable Liquid Substrate 0.577mM BCIP/0.122mM NBT

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Specifications

BCIP/NBT Stable Liquid Substrate System for Alkaline Phosphatase has been developed for the localization of AP-labeled probes on Western, Northern, Southern and Dot Blots. The system is based on hydrolysis of BCIP and reduction of NBT producing an intense purple reaction product.
Catalog #B0760
Single component substrate is more sensitive than existing products and produces little or no background staining. BCIP/NBT Stable Liquid Substrate is supplied as a liquid in an AMP (2-amino-2-methyl-1-propanol) buffer, pH 9.8.
When the phosphate group of BCIP is hydrolyzed by alkaline phosphatase (AP) the resulting indolyl derivative adds to itself forming an insoluble, blue halogenated indigo. The reaction is slow and the indigo derivative is slightly soluble in aqueous alkaline buffer solutions. If NBT is present in the system, the reaction proceeds in a different fashion.
When BCIP/NBT solution is added to a blotting system containing alkaline phosphatase labeled probes, hydrolysis of the phosphate ester by AP results in the formation of powerful reducing products. These substances reduce NBT to the insoluble NBT formazan readily visible as purple deposits at the sites of AP activity.
Note This product is not recommended for immunohistochemical or in situ hybridization techniques. For the latter methodology BCIP/NBT Enhanced is available. See Catalog# B0765.
CAS Number6578-06-9
Features
Single component system
Room temperature stable
Exhibits low background levels
Safe to use - no weighing of hazardous powders
Save preparation time - use right out of the bottle
Ultra-low sensitivity
Minimal lot-to-lot variation
Alternate names5-Bromo-4-chloro-3-indolylphosphate/Nitro Blue Tetrazolium; Staining System for AP


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