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BCIP/TNBT Stable Liquid Substrate 0.452mM/Liter BCIP:0.432mM/Liter TNBT
BCIP/TNBT Stable Liquid Substrate 0.452mM/Liter BCIP:0.432mM/Liter TNBT
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| Similar to BCIP/NBT Enhanced but is more sensitive and produces a more intense purple color. This is extremely useful in double antibody staining procedures when used with BCIP/INT where phosphatase labeled antibodies are preferred. BCIP/TNBT is supplied in a Tris HCl buffer. | | | Catalog # | B0805 | | When the phosphate group of BCIP is hydrolyzed by alkaline phosphatase (AP) the resulting indolyl derivative adds to itself forming an insoluble-blue halogenated indigo. The reaction is slow and the indigo derivative is slightly soluble in aqueous alkaline buffer solutions. If TNBT is present in the system, the reaction proceeds in a different fashion. Reducing components formed during phosphate hydrolysis react rapidly with TNBT converting it to the very insoluble dark-purple TNBT formazan. | | Features | • Single component system | | • Room temperature stable | | • Exhibits low background levels | | • Safe to use-no weighing of hazardous powders | | • Save preparation time-use right out of the bottle | | • Ultra-low sensitivity | | • Minimal lot-to-lot variation | | United States Biological provides a single component BCIP/TNBT reagent designed for immunohistochemical and in situ hybridization procedures. It may also be used for all blotting methods utilizing AP labeled reagents. | | Appearance | Colorless to pale yellow/orange | | | pH | 9.2 ± 0.05 | | | Stability Testing | 37°C, 4°C, RT | | | Performance Testing | Dot Blot | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | 5-Bromo-4-chloro-3-indolylphosphate/Tetranitrobluetetrazolium. Staining System for AP |
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