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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Bcl 2 (B Cell Leukemia 2), Pro-Survival Family Antibody Sampler Kit, BioAssay™

Bcl 2 (B Cell Leukemia 2), Pro-Survival Family Antibody Sampler Kit, BioAssay™

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Specifications

The Bcl-2 family consists of a number of evolutionarily conserved proteins containing Bcl-2
homology domains (BH) that regulate apoptosis through control of mitochondrial membrane permeability and release of cytochrome c (1-3). Four BH domains have been identified (BH1-4), which mediate protein interactions. The family can be separated into three groups
based upon function and sequence homology: pro-surivival members including Bcl-2, Bcl-xL, Mcl-1, A1, and Bcl-w; pro-apoptotic proteins including Bax, Bak, and Bok; and “BH3 only” proteins Bad, Bik, Bid, Puma, Bim, Bmf, Noxa, and Hrk. Interactions between death-promoting
and death-suppressing Bcl-2 family members has led to a rheostat model in which the ratio of pro-apoptotic and anti-apoptotic proteins controls cell fate (4). Thus, pro-survival members exert their behavior by binding to and antagonizing death-promoting members. In general, the “BH3-only members” can bind to and antagonize the pro-survival proteins leading to
increased apoptosis (5). While some redundancy of this system likely exists, tissue specificity and transcriptional and post-translational regulation of many of these family members can account for distinct physiological roles. Several phosphorylation sites have been identified within
Bcl-2 including Thr56, Ser70, Thr74, and Ser87 (6).
Catalog #B0807-02D
Included antibodiesB0807-02D1 Bcl 2, phosphorylated (Ser70) 40ul W, F H 28kD Rabbit
B0807-02D2 Bcl-2, phosphorylated (Thr56) 40ul W H 28kD Rabbit
B0807-02D3 Bcl-2 40ul W, IP, IHC H 28kD Rabbit
B0807-02D4 Bcl-XL 40ul W, IP, IHC H, M, R, Mk 30kD Rabbit
B0807-02D5 Mcl-1 40ul W H 40kD Rabbit
B0807-02D6IgG (HRP) 100ul Goat
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PurityProtein A and peptide affinity chromatography.
Concentration5x40ul
(4 Western mini-blots)
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol.
SpecificityEach antibody in the Pro- Survival Bcl-2 Family Antibody Sampler Kit (Human Specific) recognizes only its specific target. The antibodies do not cross-react with other Bcl-2 family members.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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