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You are here:Home » Molecular Biology » MB-Infectious Disease » Bcl 2 (BCL2, Bcl-2, B-cell CLL/lymphoma 2) (Control Peptide)

Bcl 2 (BCL2, Bcl-2, B-cell CLL/lymphoma 2) (Control Peptide)

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Specifications

AGRTGYDNREIVMKYC located at the N Terminus of the protein sequence.
Control peptide corresponding to polyclonal antibody, Catalog#B0807-02E, goat x human.
Catalog #B0807-02E-P
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilution to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide Blocking (see corresponding antibody B0807-02E)Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
PurityHighly Purified
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in PBS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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