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You are here:Home » Antibodies » Abs to Apoptosis » Anti -Bcl 2 (B Cell Leukemia 2)

Anti -Bcl 2 (B Cell Leukemia 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Catalog #B0807-02J
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot (ECL): 5ug/ml; Titration is recommended for optimal results in individual systems.
Immunohistochemistry (Parrafin): 2.5ug/ml; Pressure cooker antigen retrieval is recommended. Use DAB detection
Immunohistochemistry (Frozen): 5ug/ml; No pre-treatment needed. Use DAB detection.
Optimal dilutions to be determined by the researcher.
Western Blot ControlsPositive: MCF-7 cells
Negative: Normal rabbit IgG
IHC ControlsPositive: Intestine or human lymph node
Negative: Brain tissue (only random cells will stain for Bcl-2)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa20-34 of human Bcl 2.
SpecificityRecognizes human Bcl 2. Particularly useful for the determination of Bcl 2 expression levels in species other than human. Species Crossreactivity: Mouse and opossum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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