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You are here:Home » Antibodies » Abs to Cancer Markers, Leukemia » Anti -Bcl-2 (B Cell Leukemia 2)

Anti -Bcl-2 (B Cell Leukemia 2)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
Apoptosis regulator Bcl-2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. At least 2 isoforms (alpha and beta) are produced by alternate splicing with different C-terminal ends.
Catalog #B0807-06C
ApplicationsSuitable for use in Immunohistochemistry and Western Blot . Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
Immunohistochemistry (formalin, paraffin, and frozen): 0.4-1.0ug/ml
Antigen retrieval may improve the staining.
Optimal dilution determined by the researcher.
Positive controlFollicular and diffuse large B cell lymphoma tumors
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9G317
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide (GAAPAPGIFSSQPG) corresponding to aa41-54 from human Bcl-2 conjugated to thyroglobin.
SpecificityRecognizes human Bcl-2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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