Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IH

Apoptosis regulator Bcl-2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. At least 2 isoforms (alpha and beta) are produced by alternate splicing with different C-terminal ends.
Catalog #	B0807-06C
Applications	Suitable for use in Immunohistochemistry and Western Blot . Other applications not tested.
Recommended Dilution	Western Blot: 1-2ug/ml
	Immunohistochemistry (formalin, paraffin, and frozen): 0.4-1.0ug/ml
	Antigen retrieval may improve the staining.
	Optimal dilution determined by the researcher.
Positive control	Follicular and diffuse large B cell lymphoma tumors
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9G317
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
Purity	Purified by affinity chromatography.
Immunogen	Synthetic peptide (GAAPAPGIFSSQPG) corresponding to aa41-54 from human Bcl-2 conjugated to thyroglobin.
Specificity	Recognizes human Bcl-2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.