| Human/mouse/rat Bcl-2 specific PCR primers for RT-PCR (reverse transcription followed by |
|
| polymerase chain reaction) analysis of mRNA expression. |
|
| Catalog # | B0807-06D |
| Components | Primer Pair: Lyophilized. Each vial contains 375pmoles of each primer. Adjust to a final concentration of 7.5 pmoles/ul by resuspending Primer Pair in either 50ul autoclaved deionized water or 0.1X TE buffer (1mM Tris HCl, pH8.0 @ 25°C; 0.1mM EDTA, pH 8.0 @ 25°C). |
| Positive Control 3 | Lyophilized. Each vial contains 150 ng synthetic double-stranded DNA. Resuspend in 30ul autoclaved deionized water or TE buffer. To prevent contaminating samples and reagents, it is strongly recommended that the Positive Control is resuspended in a separate location from where PCR reactions are set up. Use a different pipette than one used for PCR set up. The Positive Control is not intended for quantitative purposes. |
| GenBank Accession Number | M13994, M16506, U34964 for human, mouse, and rat, respectively. Bcl-2 primer sequences are completely conserved among human, mouse and rat |
| Product Sizes | Bcl-2 primer sequences are completely conserved among human, mouse and rat. |
| cDNA: 293 base pairs (bp) |
| Genomic DNA: None observed. |
| Positive Control 3: 340 bp |
| Alternate splice variants: Yes, only alpha is amplified. |
| Suggested Reaction Conditions | For each 50ul Polymerase Chain Reaction mixture, 2ul of the primer pair stock solution should be used. Sufficient reagent is supplied for 25 separate amplification reactions of the gene fragment. |
| Thermocycling Conditions | A typical 50ul PCR would involve a 4 minute denaturation step at 94ºC, followed by 30-35 cycles of 45 seconds at 94ºC, 45 seconds at 55ºC and 45 seconds at 72ºC, with a final 10 minute extension step at 72ºC. |
| Storage and Stability | The Primer Pair resuspended in water or TE buffer is stable for up to one year at -20°C in a non-frost free freezer. Aliquot in single use portions. Do not use past the expiration date above. Avoid repeated freeze-thaw cycles. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
