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You are here:Home » Antibodies » Abs to Cancer Markers, Leukemia » Anti -Bcl-2 (B Cell Leukemia 2)

Anti -Bcl-2 (B Cell Leukemia 2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
The human bcl-2 gene family plays a central role in lymphoid system cell survival. Continuous expression of bcl-2 protein is critical for preventing apoptosis of mature peripheral B and T cells.
Catalog #B0807-06E
ApplicationsSuitable for use Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 5mg/ml 80ul labels 5x10e5 cells
Optimal dilutions to be determined by the researcher.
FACS Protocol1. Cultured Nalm-6 human tumor cells were fixed for 30 minutes with 2% buffered formaldehyde after which they were washed two times.
2. Subsequent reagent incubations and washes were done using a buffer containing 0.3% Saponin to permeabolize cells. 3. Five x 10e5 cells per tube were preincubated ~5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding).
4. They were then incubated for 45 minutes on ice with 80ul of B0807-06E at 5ug/ml.
5. Cells were then washed twice and incubated with 2º reagent IgG (FITC) PAB Gt xMo then washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained with a mean shift of 0.56 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Recommended Secondary AntibodiesI1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo
I1905-18D IgM (X-Adsorbed) (FITC) Pab GtxMo
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No7H161
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.5mg/ml gentamicin sulfate.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman bcl-2 peptide 41-54
SpecificityRecognizes the intracellular human bcl-2 protein at ~26kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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