| The human bcl-2 gene family plays a central role in lymphoid system cell survival. Continuous expression of bcl-2 protein is critical for preventing apoptosis of mature peripheral B and T cells. |
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| Catalog # | B0807-06E |
| Applications | Suitable for use Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: 5mg/ml 80ul labels 5x10e5 cells |
| Optimal dilutions to be determined by the researcher. |
| FACS Protocol | 1. Cultured Nalm-6 human tumor cells were fixed for 30 minutes with 2% buffered formaldehyde after which they were washed two times. |
| 2. Subsequent reagent incubations and washes were done using a buffer containing 0.3% Saponin to permeabolize cells. 3. Five x 10e5 cells per tube were preincubated ~5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding). |
| 4. They were then incubated for 45 minutes on ice with 80ul of B0807-06E at 5ug/ml. |
| 5. Cells were then washed twice and incubated with 2º reagent IgG (FITC) PAB Gt xMo then washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained with a mean shift of 0.56 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. |
| Recommended Secondary Antibodies | I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo |
| I1905-18D IgM (X-Adsorbed) (FITC) Pab GtxMo |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 7H161 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, 0.5mg/ml gentamicin sulfate. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Human bcl-2 peptide 41-54 |
| Specificity | Recognizes the intracellular human bcl-2 protein at ~26kD. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
