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You are here:Home » Antibodies » Abs to Cancer Markers, Leukemia » Anti -Bcl-2 (B Cell Leukemia 2) (Biotin)

Anti -Bcl-2 (B Cell Leukemia 2) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
The human bcl-2 gene family plays a central role in lymphoid system cell survival. Continuous expression of bcl-2 protein is critical for preventing apoptosis of mature peripheral B and T cells.
Catalog #B0807-06F
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 5ug/ml; 80ul labels 5x10e5 Nalm-6 tumor cells
Immunohistochemistry: Fixed, paraffin sections
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. Cultured Nalm-6 human tumor cells were fixed for 30 min with 2% buffered formaldehyde after which they were washed two times.
2. Subsequent reagent incubations and washes were done using a buffer containing 0.3% Saponin to permeabolize cells.
3. Five x 10e5 cells per tube were incubated 45 minutes on ice with 80ul of B0807-06F at 5ug/ml.
4. Cells were then washed twice and incubated with S7973-88P Streptavidin (PE).
5. Cells were washed three times, fixed and analyzed by FACS.
6. Cells stained with a mean shift of 0.44 log10 fluorescent units when compared to a Mouse IgG1 negative control (Biotin) at a similar concentration.
7. This shift was blocked when permeabalized cells were preincubated 10 minutes with 20ul of 0.5mg/ml B0807-06E.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No7H161
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa41-54 of human Bcl-2.
SpecificityRecognizes the intracellular human Bcl-2 protein at ~26kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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