| The human bcl-2 gene family plays a central role in lymphoid system cell survival. Continuous expression of bcl-2 protein is critical for preventing apoptosis of mature peripheral B and T cells. |
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| Catalog # | B0807-06F |
| Applications | Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Flow Cytometry: 5ug/ml; 80ul labels 5x10e5 Nalm-6 tumor cells |
| Immunohistochemistry: Fixed, paraffin sections |
| Optimal dilutions to be determined by the researcher. |
| Flow Cytometry Protocol | 1. Cultured Nalm-6 human tumor cells were fixed for 30 min with 2% buffered formaldehyde after which they were washed two times. |
| 2. Subsequent reagent incubations and washes were done using a buffer containing 0.3% Saponin to permeabolize cells. |
| 3. Five x 10e5 cells per tube were incubated 45 minutes on ice with 80ul of B0807-06F at 5ug/ml. |
| 4. Cells were then washed twice and incubated with S7973-88P Streptavidin (PE). |
| 5. Cells were washed three times, fixed and analyzed by FACS. |
| 6. Cells stained with a mean shift of 0.44 log10 fluorescent units when compared to a Mouse IgG1 negative control (Biotin) at a similar concentration. |
| 7. This shift was blocked when permeabolized cells were preincubated 10 minutes with 20ul of 0.5mg/ml B0807-06E. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 7H161 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa41-54 of human Bcl-2. |
| Specificity | Recognizes the intracellular human Bcl-2 protein at ~26kD. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
