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You are here:Home » Antibodies » Abs to Proteins » Anti -Bcl 2 Inhibitor of Transcription 1 (Bit1, Peptidyl-tRNA Hydrolase 2)

Anti -Bcl 2 Inhibitor of Transcription 1 (Bit1, Peptidyl-tRNA Hydrolase 2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the ?5?1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the ?5?1 integrin.
Catalog #B0807-09U1
Positive Control Daudi cell lysate can be used as positive control.
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
PurityPurified by peptide immunoaffinity chromatography.
ImmunogenHuman Bit1 / Bcl-2 inhibitor of transcription 1 / Peptidyl-tRNA hydrolase 2 (IN) peptide.
SpecificitySpecies Crossreactivity: Human, Mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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