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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » BcnI (CauII)

BcnI (CauII)

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Specifications

G
5'-C C^C G G-3'
3'-G G G^C C-5'
C
Catalog #B0808
Concentration 10u/ul
Source Bacillus centrosporus RFL1
Buffer 33mM Tris-acetate (pH 7.9), 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA. Incubate at 37°C.
Diluent Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods–the Storage Buffer should be used.
Storage Buffer 10mM potassium phosphate (pH 7.4 at 25°C), 200mM NaCl, 1mM EDTA, 7mM 2-mercaptoethanol, 0.2mg/ml BSA and 50% glycerol.
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BcnI.
Ligation/Recutting AssayAfter 10-fold overdigestion (2u/ug DNA x 5 hours) with BcnI, more than 80% of the DNA fragments can be ligated in a reaction mixture containing 20–40u of T4 DNA ligase/1ug of fragments and 10% PEG at a 5'-termini concentration of 0.03uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Methylation EffectsSequence 5'…Cm5CGGG…3' 3'…GGm5CCC…5' is cleaved by BcnI at a significantly slower rate. CG methylation may influence DNA cleavage.
Stability during Prolonged IncubationA minimum of 0.2units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Thermal InactivationEnzyme is inactivated by incubation at 65°C for 20min.
Compatible EndsBme1390I, SatI.
Number of Recognition Sites in DNA
Lambda114
PhiX1741
M13mp18/194
pBR32210
pUC18/197
pUC577
pTZ19R/U5
pBluescriptIIKS(-/+)6
pBluescriptIISK(-/+)6
pACYC1776
pACYC18410
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.


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