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You are here:Home » Kits and Assays » Kits for ELISA, BioAssay™ » Beta Defensin 1, Human, BioAssay™ ELISA Kit (Beta-defensin 1, BD-1, hBD-1, Defensin, beta 1, DEFB1, BD1, HBD1)

Beta Defensin 1, Human, BioAssay™ ELISA Kit (Beta-defensin 1, BD-1, hBD-1, Defensin, beta
1, DEFB1, BD1, HBD1)

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Specifications

Defensins are cationic, anti-microbial peptides, produced by many cell types (e.g., leukocytes, epithelium, dendrites), and which play prominent roles in the innate immune response of mammals. Two classes described in humans, the alpha- and beta-defensins, range from 3.5 to 4.5kD and are stabilized by three intramolecular disulfide bonds differing by the ordering of the disulfide bonds in the mature peptides. To date, six alpha-defensins (also referred to as Human Neutrophil Peptides, HNP1-6) and six beta-defensins (HBD1-6) have been investigated. All have demonstrated broad-spectrum in vitro antimicrobial activity against bacteria, fungi and enveloped viruses – activities that are expected to be significant for in vivo protection against pathogens. Researchers have also investigated the ability of defensins to induce the release from tissues of cytokines and chemokines involved in inflammatory responses, thereby providing a regulatory role that bridges innate and adaptive immunity. Investigative research has led to the measurement of defensins in serum, saliva, milk, amniotic fluid and lung and cervicovaginal lavage, and culture media of cells and tissue of blood, lung, skin, bowel, muscle, cartilage and kidney, among other sample types.
Catalog #B0900-31
Sample TypeSerum, saliva, milk, amniotic fluid and lung and cervicovaginal lavage, and culture media of cells and tissue of blood, lung, skin, bowel, muscle, cartilage and kidney, among other sample types.
Intended UseThe Human Beta Defensin 1 (BD-1) ELISA Kit is an in vitro immunoassay for research use in the quantification of BD-1 in cultures of human cells and in appropriately qualified samples from serum, saliva or other tissue fluids.
RangeHuman saliva: 9.4-23.4ng/ml.
Culture Medium: BD-1 was spiked into Sample Diluent with 10% Neonatal Bovine Serum at 4 levels, 100-800pg/ml. The mean recovery ranged from 105-118%, demonstrating linear dilution and equivalent quantification across the standard range.
Test PrincipleThe Human Beta Defensin 1 ELISA kit is based on the binding of Human Beta Defensin 1 in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to biotin, which then binds to a streptavidin horseradish peroxidase (HRP) conjugate. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of BD-1 present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of BD-1 in samples is calculated from a standard curve of purified recombinant human BD-1 of designated concentration.
Kit ComponentsB0900-31A: Microtiter Strip Plate 1x96 wells coated with Human BD1 AB
B0900-31B: Standard 800pg/ml 3x1vial
B0900-31C: Ab (Biotin), 100X 1x150ul
B0900-31D: Streptavidin (HRP), 100X 1x150ul
B0900-31E: Sample Diluent, 20X 1x10ml
B0900-31F: Wash Solution, 100X 1x10ml
B0900-31G: TMB Substrate 1x12ml
B0900-31H: Stop Solution (1% H2SO4) 1x12ml
Storage and StabilityStore components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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