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You are here:Home » Antibodies » Abs to Defensin » Anti -BD3 (beta Defensin-3)

Anti -BD3 (beta Defensin-3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Beta Defensin-3 is a novel 5-9kD nonhemolytic antimicrobial protein peptide which may be important in the innate epithelial defense of infections by various microorganisms seen in skin and lung (such as cystic fibrosis).
Catalog #B0902
ApplicationsSuitable for use Western Blot. Other applications not tested.
Recommended DilutionWestern Blot:
Tricine-SDS-PAGE gels: 1:500-1:1000. Migrates as a 5-6kD band.
Tricine-SDS-urea PAGE gels: Native Beta Defensin 3 migrates as a 9kD band. The larger size is because of the reduction of the three cysteine bridges present in the peptide protein.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide consisting of aa 23-33 (GIINTLQKYYC) of the human beta-defensin-3 protein
SpecificityRecognizes the 5kD human Defensin-3, beta protein. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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