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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

Beta Defensin-3 is a novel 5-9kD nonhemolytic antimicrobial protein peptide which may be important in the innate epithelial defense of infections by various microorganisms seen in skin and lung (such as cystic fibrosis).
Catalog #	B0902
Applications	Suitable for use Western Blot. Other applications not tested.
Recommended Dilution	Western Blot:
	Tricine-SDS-PAGE gels: 1:500-1:1000. Migrates as a 5-6kD band.
	Tricine-SDS-urea PAGE gels: Native Beta Defensin 3 migrates as a 9kD band. The larger size is because of the reduction of the three cysteine bridges present in the peptide protein.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 0.02% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	A synthetic peptide consisting of aa 23-33 (GIINTLQKYYC) of the human beta-defensin-3 protein
Specificity	Recognizes the 5kD human Defensin-3, beta protein. Species Crossreactivity: human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.