BD3, Recombinant, Human (beta Defensin-3)
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| Recombinant Human beta-defensin-3 (BD-3) in an antimicrobial peptide, which belongs to the distinct family of beta-defenisns. Recombinant Human BD-3 is a 5.1kD protein containing 45 amino acid residues. | | | Catalog # | B0902-01 | | Beta-Defensin 3, also known as BD3 and DEFB-3, is a membrane-active cationic peptide that functions in inflammation and innate immune responses. There are at least 30 beta-Defensins which are distinguished from alpha-Defensins by the connectivity pattern of their three intramolecular disulfide bonds (1). The 45 aa mature human BD3 shares 38% and 33% aa sequence identity with mouse and rat BD3, respectively (2, 3). It shares 18%-36% aa sequence identity with other human beta-Defensins. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is upregulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds (2, 4-6). BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 production and inhibits TIMP1 and 2 expression (7). In vivo control of BD3 activity is accomplished in part through cleavage by cathepsins B, L, and S (8). BD3 displays strain specific microbicidal activity toward a broad spectrum of bacteria and yeast (2, 9). BD3 also induces monocyte migration, mast cell activation, and a mast cell-dependent increase in vascular permeability (4, 10). Disruption of the intramolecular disulfide bond pattern in BD3 abrogates its monocyte chemoattractant properties but not its antimicrobial properties (11, 12). BD3 inhibits viral infectivity by interacting directly with HIV-1 plus its coreceptor CXCR4 (5, 13), and with HSV glycoprotein B plus its receptor heparan sulfate (14), and by forming a protective coating on the surface of influenza virus target cells (15). | | Biological Activity | Exhibits antimicrobial activity against gram-positive bacteria S. aureus and gram-negative P. aeruginosa and E. coli. | | AA Sequence | GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK | | Storage and Stability | Reconstitute with acetic acid, aliquot and store at -20°C. Stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 5.1kD | | Source | E. coli (Human, recombinant). | | Purity | 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug) | | Form | Supplied as a sterile-filtered, lyophilized powder. Reconstitute in 10mM acetic acid to 0.1-1mg/ml. THIS SOLUTION CAN THEN BE DILUTED INTO OTHER AQUEOUS SOLUTIONS with carrier protein.. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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