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You are here:Home » Antibodies » Abs to Defensin » Anti -BD3 (beta-Defensin 3)

Anti -BD3 (beta-Defensin 3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms. The genes encoding human alpha- and beta-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated alpha– and beta- based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six alpha–defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two beta-defensins, HBD-1 and HBD-2, have been identified to date. Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologs of the human beta-defensin have also been identified.
Catalog #B0902-03C
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000-1:5000 (ECL)
ELISA: 1:10,000-1:50,000; Control peptide (Cat #B0902B) can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot Determined
FormSupplied as a lyophillized powder from 0.05% sodium azide. Reconstitute with 100ul PBS, pH 7.4.
PuritySerum
ImmunogenA synthetic peptide corresponding to 13aa at the N-terminus of mature mouse defensin-3 and a 15aa peptide mapping at the N-terminus of mature human defensin-3. The two peptides were mixed.
SpecificityRecognizes defensin-3. Species Crossreactivity: mouse and human. Does not recognize other beta-defensins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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