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You are here:Home » Antibodies » Abs to Defensin » Anti -BD3 (beta-Defensin 3) (Biotin)

Anti -BD3 (beta-Defensin 3) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #B0902-03E
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA (Direct, Sandwich): 0.25-1.0ug/ml Detects 0.2ng/well of recombinant hBD-3 using 100ul/well antibody solution in conjunction with B0902-03D as a capture antibody. Allows the detection of 0.2-0.4ng/well of recombinant hBD-3.
Western Blot: 0.1-0.2ug/ml (under either reducing or non-reducing conditions) Detection limit for recombinant hBD-3 is 1.5-3.0ng/lane.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS, 0.1% BSA.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a lyophilized powder from PBS, pH 7.2. Conjugated to Biotin. Reconstitute with sterile 40-50% glycerol, PBS, 0.1% BSA to a concentration of 0.1-1.0mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly pure (> 98%) recombinant human Beta Defensin-3
SpecificityRecognizes human Beta Defensin-3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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