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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Catalog #	B0902-03E
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA (Direct, Sandwich): 0.25-1.0ug/ml Detects 0.2ng/well of recombinant hBD-3 using 100ul/well antibody solution in conjunction with B0902-03D as a capture antibody. Allows the detection of 0.2-0.4ng/well of recombinant hBD-3.
	Western Blot: 0.1-0.2ug/ml (under either reducing or non-reducing conditions) Detection limit for recombinant hBD-3 is 1.5-3.0ng/lane.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS, 0.1% BSA.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not Determined
Form	Supplied as a lyophilized powder from PBS, pH 7.2. Conjugated to Biotin. Reconstitute with sterile 40-50% glycerol, PBS, 0.1% BSA to a concentration of 0.1-1.0mg/ml.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Highly pure (> 98%) recombinant human Beta Defensin-3
Specificity	Recognizes human Beta Defensin-3.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.