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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Defensin beta 4 (BD4), a secreted cationic antimicrobial peptide and member of the beta-defensin family, highly expressed in the testis, which plays an important role in innate immunity. Studies have shown that the expression of BD-4 is up-regulated in respiratory epithelial cells, following infection by both gram-positive and gram-negative bacteria, and that BD-4 acts in synergy with lysozyme and BD-3.
Catalog #	B0903-25B
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 0.25-1ug/ml
	Western Blot: 0.1- 0.2ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a lyophilized powder in PBS, pH7.2. Labeled with Biotin. Reconstitute with 500ul sterile PBS, 0.1% BSA.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Recombinant human defensin beta 4.
Specificity	Recognizes human defensin beta 4 (BD4).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.