Anti -Beclin-1 (Beclin 1 (Coiled-coil Moesin-like BCL2-interacting Protein), Beclin1,
BECN1, APG6, ATG6, ATG6 Autophagy-related 6 Homolog, Coiled-coil Myosin-like
Bcl-2-Interacting Protein, GT197, Protein GT197, VPS30)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B |
|
| Beclin1 is a 60kD member of the Beclin family of proteins. It is part of a lipid-kinase complex that includes UVRAG and PI3 and induces autophagy; in the presence of Bcl-2, autophagy is repressed. Beclin1 is widely expressed but abundantly found in mammary epithelium, glia and neurons. Human Beclin1 is 450aa in length and contains a Bcl-2 binding domain aa88-150, a nuclear export signal motif aa180-189, a coiled-coil region aa142-270, and an evolutionary conserved domain aa244-337. There is one potential alternate start site at Met88. Over aa12-239, human Beclin1 is 98aa identical to mouse Beclin1. | | | Catalog # | B0981-23S | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2B | | Clone No | 11n13 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml. | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Recombinant corresponding to aa12-239 from human Beclin1 expressed in E. coli (Q14457). | | Specificity | Recognizes human Beclin1, human, mouse and rat. No cross-reactivity with recombinant human ATG3, 4A, 4B, 5, 7, 10, 12, or 16L1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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