Anti -Beclin-1 (Beclin 1 (Coiled-coil Moesin-like BCL2-interacting Protein), Beclin1,
BECN1, APG6, ATG6, ATG6 Autophagy-related 6 Homolog, Coiled-coil Myosin-like
Bcl-2-Interacting Protein, GT197, Protein GT197, VPS30) (HRP)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IH IF |
|
| Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses. | | | Catalog # | B0981-23T | | Applications | Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry (paraffin). Other applications not tested. | | Recommended Dilution | Western Blot: 1:2000-1:5000, a band is seen at ~52 kD | | Immunofluorescence: mouse tissue | | Optimal dilution determined by the researcher. | | Storage and Stability | Store at 2-8°C. Do Not Freeze! Aliquots are stable for at least 3 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in PBS. Conjugated to HRP. No preservative added. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Internal synthetic peptide to human Beclin 1, containing residues within 1-100. Cellular Localization: Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. | | Specificity | Recognizes human Beclin 1. 94% identity with the mouse sequence and 88% identity with the rat sequence. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Beclin 1 Autophagy-related |
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