| BetaCellulin is a Type I membrane protein in precursor form but an extracellular protein in mature form. It is Expressed in a wide range of tissues, including the mammary gland. BetaCellulin is a Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. The effects of betacellulin are probably mediated by the EGF receptor and other related receptors. |
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| Recombinant Bovine Betacellulin produced in E.coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and having a molecular mass of 9003 Dalton. |
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| Recombinant Bovine BTC is purified by proprietary chromatographic techniques. |
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| Catalog # | B1081-40 |
| Solubility | It is recommended to reconstitute the lyophilized Bovine BTC in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Gly-Asn-Ser-Thr. |
| Dimers and aggregates | 1% as determined by silver-stained SDS-PAGE gel analysis. |
| Biological Activity | Fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is < 10.0ng/ml. |
| Endotoxin | 0.1ng/ug (IEU/ug) of Bovine Betacellulin. |
| Protein content | Protein quantitation was carried out by two independent methods: |
| 1. UV spectroscopy at 280nm using the absorbency value of 0.59 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). |
| 2. Analysis by RP-HPLC, using a calibrated solution of BTC as a Reference Standard. |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Molecular Weight | 9kD |
| Source | E. coli |
| Purity | 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Concentration | As reported |
| Form | Supplied as a lyophilized powder from 50mM acetic acid. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
