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You are here:Home » Molecular Biology » Growth Factors-EGF » Betacellulin, Recombinant, Bovine (BTC, Probetacellulin)

Betacellulin, Recombinant, Bovine (BTC, Probetacellulin)

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Specifications

BetaCellulin is a Type I membrane protein in precursor form but an extracellular protein in mature form. It is Expressed in a wide range of tissues, including the mammary gland. BetaCellulin is a Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. The effects of betacellulin are probably mediated by the EGF receptor and other related receptors.
Recombinant Bovine Betacellulin produced in E.coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and having a molecular mass of 9003 Dalton.
Recombinant Bovine BTC is purified by proprietary chromatographic techniques.
Catalog #B1081-40
SolubilityIt is recommended to reconstitute the lyophilized Bovine BTC in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Amino acid sequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Asp-Gly-Asn-Ser-Thr.
Dimers and aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityFully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is < 10.0ng/ml.
Endotoxin 0.1ng/ug (IEU/ug) of Bovine Betacellulin.
Protein contentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.59 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of BTC as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight9kD
SourceE. coli
Purity95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
ConcentrationAs reported
FormSupplied as a lyophilized powder from 50mM acetic acid.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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