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You are here:Home » Antibodies » Abs to Enzymes, Methyltransferase » Anti -Betaine-Homocysteine Methyltransferase (BHMT, Betaine-Homocysteine S-Methyltransferase)

Anti -Betaine-Homocysteine Methyltransferase (BHMT, Betaine-Homocysteine
S-Methyltransferase)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulfur amino acids, choline and betaine.
Catalog #B1082-12B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from BALB/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No7G21
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human BHMT (1-406aa) (E. coli)
SpecificityRecognizes human BHMT at ~45kD. Species Crossreactivity: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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