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Anti -Betaine-homocysteine S-methyltransferase, CT (BHMT)
Anti -Betaine-homocysteine S-methyltransferase, CT (BHMT)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IH |
|
| Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulfur aa, choline and betaine. | | | Catalog # | B1082-12F | | Applications | Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | ELISA: 1:16,000 | | Western Blot: 1:16,000, 0.1-0.3ug/ml | | Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to C-EQQLKELFEKQK from human BHMT at C-terminal. | | Specificity | Recognizes human BHMT. Species sequence homology: Canine, mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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