Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

5'-C^T Pu Py A G-3'
3'-G A Py Pu T^C-5'
Catalog #	B1086
Concentration	10u/ul
Source	Bacillus firmus S8–336
Buffer	33mM Tris-acetate (pH 7.9), 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA. Incubate at 37°C.
Diluent Buffer	10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage Buffer	10mM Tris-HCl (pH 7.5 at 25°C), 100mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion Assay	No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with BfmI (see Star Activity).
Ligation/Recutting Assay	After 50-fold overdigestion (3u/ug DNA x 17 hours) with BfmI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.5uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) Assay	No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Star Activity	A large excess of enzyme (7.5u/ug DNA x 16 hours) may result in star activity.
Stability during Prolonged Incubation	A minimum of 1.0unit of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Thermal Inactivation	Enzyme is inactivated by incubation at 65°C for 20min.
Compatible Ends	C^TGCAG–Alw44I
Number of Recognition Sites in DNA
Lambda	38
PhiX174	6
M13mp18/19	7
pBR322	4
pUC18/19	4
pUC57	4
pTZ19R/U	6
pBluescriptIIKS(-/+)	6
pBluescriptIISK(-/+)	6
pACYC177	1
pACYC184	1
Unit Definition	One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.