Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

5'-G C C N N N N^N G G C-3'
3'-C G G N^N N N N C C G-5'
Catalog #	B1087
Concentration	10u/ul
Source	Bacillus globigii
Buffer	50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA Incubate at 37°C.
Diluent Buffer	10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods–the Storage Buffer should be used.
Storage Buffer	10mM Tris-HCl (pH 7.5 at 25°C), 300mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion Assay	No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BglI.
Ligation/Recutting Assay	After 50-fold overdigestion (3u/ug DNA x 17 hours) with BglI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.3uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) Assay	No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Methylation Effects	The hemi-methylated sequence GCCN5GGm5C is cleaved by BglI at a significantly slower rate. Cleavage is blocked when both strands are methylated at positions: 5'… GCCN5GGm5C…3' 3'…m5CGGN5CC G…5'. Overlapping CG methylation may influence DNA cleavage.
Stability during Prolonged Incubation	A minimum of 0.1units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Thermal Inactivation	Enzyme is inactivated by incubation at 65°C for 20min.
Digestion of Agarose-embedded DNA	A minimum of 5units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Number of Recognition Sites in DNA
Lambda	29
PhiX174	0
M13mp18/19	1
pBR322	3
pUC18/19	2
pUC57	2
pTZ19R/U	2
pBluescriptIIKS(-/+)	2
pBluescriptIISK(-/+)	2
pACYC177	1
pACYC184	2
Unit Definition	One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.