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Specifications

Differentiation of Group D from non-Group D Streptococci.
Catalog #	B1099
Description	Bile Esculin Agar is prepared according to the formula specified by Swan, and later evaluated by Facklam and Moody. It was concluded that the bile esculin test provided a reliable means of identifying enterococci and differentiation from non-enterococci. Enterococci are bile tolerant and hydrolyze esculin which imparts a dark brown color to the medium. Gram positive bacteria other than enterococci are inhibited by the bile salts.
Storage	Store sealed bottle containing dehydrated medium at 2 to 30°C. Once opened and recapped, place container in low humidity environment at same storage temperature. Protect from moisture and light. Dehydrated medium should be discarded if not free flowing or if color has changed from original light beige color.
Microbiological Analysis
Escherichia coli (ATCC 25922)	Growth, Esculin (+)
Enterococcus faecalis (ATCC19433)	Growth, Esculin (+)
Enterococcus faecalis (ATCC 29212)	Growth, Esculin (+)
Enterococcus faecalis (ATCC33186)	Growth, Esculin (+)
Streptococcus pyogenes (ATCC 19615)	Inhibited, Esculin (-)
Appearance	Light beige, homogeneous and free flowing
Solubility	Dark amber and turbid to clear.
pH	6.6 ± 0.2
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.