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Specifications

Bile Salts Mixture #3 is a mixture of sodium cholate and deoxycholate derived from liver. It’s primary use is as a selective agent for the isolation of gram-negative microorganisms, inhibiting gram-positive cocci. It is soluble in distilled or dionized water and neutral to slightly alkaline.
Catalog #	B1099-09
Prepared Appearance (2% wt/vol)	Clear, colorless to light amber, precipitate-free solution.
pH (2% solution at 25deg.C)	7.8 - 8.6
Expected Cultural Response
E. coli (ATCC 25922)	Good to excellent growth, pink to red colonies surrounded by a zone of precipitated bile salts.
Staphylococcus aureus (ATCC 25923)	Complete inhibition.
Test procedure	Refer to references below.