| Bin1 is a ubiquitously expressed protein that interacts with the N-terminal region of the oncoprotein Myc. It inhibits cell transformation by Myc and has been implicted in Myc-mediated apoptosis. Bin1 is structurally related to amphiphysin, a breast cancer associated autoimmune antigen, and RVS167, a negative regulator of cell cycle in yeast. Bin1 is frequently absent in carcinoma cells and the gene maps to a hot spot for deletion in prostate cancer. Thus Bin1 has the features of a tumor suppressor and may play a role in apoptosis or cell cycle regulation. |
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| Catalog # | B1200 |
| Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitaton and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilution | Western Blot: 0.5-2 ug/ml detects Bin1 in a RIPA lysate of cells. |
| Immunohistochemistry: Gives positive staining for Bin1 in C2C12, LNCap, HeLa, and Rat1 cells. |
| Optimal dilutions to be determined by the researcher. |
| *Recommended secondary antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 0.T.07 |
| Host | Mouse |
| Source | Human |
| Concentration | 1mg/ml |
| Form | Supplied as solution in 0.1M Tris-glycine, pH 7.4, containing 0.05% sodium azide. |
| Purity | Protein G purified |
| Immunogen | Partial fusion protein corresponding to amino acids 189-398 of human Bin1. |
| Specificity | Recognizes Bin1 of approximately 56kD and another protein of approximately 75kD, which may be the phosphorylated form of Bin1. Species Crossreactivity: Rat and mouse. |
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| Important Note | This product is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
