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You are here:Home » Antibodies » Abs to Vitamins » Anti -Biotin (FITC)

Anti -Biotin (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified IH IC FC
Catalog #B1750-01M
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 1:20-1:2000
Immunocytochemistry: 1:50-1:5000
Optimal dilutions to be determined by the researcher.
Absorption Wavelength 494nm
Emission Wavelength: 518nm
Fluorochrome/Protein Ratio 8.1 moles FITC per mole of Goat IgG
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostGoat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1% BSA (IgG and protease free), 0.05% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified by affinity chromatography.
ImmunogenBiotin
SpecificityRecognizes free biotin, coupled with KLH, BSA or IgG/Y. No reactivity to KLH or BSA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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