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You are here:Home » Antibodies » Abs to Vitamins » Anti -Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm)

Anti -Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B FC
Catalog #B1750-06
ApplicationsSuitable for use in Confocal Microscopy, ELISA, Flow Cytometry, Image Analysis, In Situ Nucleic Acid Hybridization and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5ug/ml for 2 hrs at RT.
Optimal dilutions to be determined by the researcher.
Note In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
SourceAscites
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.30
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%. Also available without BSA and azide. See B1750-06X.
PurityPurified by Protein G affinity chromatography.
ImmunogenBiotin (BSA coupled).
SpecificityRecognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. Species Crossreactivity: All species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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