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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm) (AP)

Anti -Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm) (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #B1750-15
ApplicationsSuitable for use in ELISA, Western and Dot Blot, Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays. Other applications have not been tested.
Recommended DilutionELISA: 1:1000 to 1:5000
Western Blot: 1:500-1:2500
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityStore at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Clone TypePolyclonal
IsotypeIgG
HostGoat
Concentration~0.9mg/ml
FormSupplied as a liquid in 0.05M Tris Chloride, 0.15M sodium chloride, 0.001M Magnesium Chlorid, 0.0001M zinc chloride, 50% glycerol, 10mg/ml BSA, and 0.01% sodium azide. Labeled with alkaline phosphatase.
PurityPurified by immunoaf nity chromatography.
ImmunogenBiotin (KLH).
SpecificityRecognizesBiotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum and Biotinylated IgG.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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